Iodination of Escherichia coli with chloramine T: selective labeling of the outer membrane lipoprotein.
نویسندگان
چکیده
Iodination of Escherichia coli cells with chloramine T preferentially labels the free and murein-bound forms of the outer membrane lipoprotein. Iodination for 15 s at 15 degrees C labels the two forms of the lipoprotein almost exclusively, whereas iodination for 60 s at 25 degrees C also labels the other major outer membrane proteins. Chloramine T iodination is a rapid, simple technique for labeling the outer membrane lipoprotein.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 130 2 شماره
صفحات -
تاریخ انتشار 1977